How We Got Here

 

Instrumentation at JR PETERS Lab

Prodigy XP ICP 

Prodigy XP ICP manufactured by Teledyne Leeman Labs

P and K are determined by our ICP (inductively couple argon plasma) Spectrometer. This instrument measures a number of elements. Here at J.R. Peters Lab we routinely test for Al, B, Ca, Cu, Fe, K, Mg, Mn, Mo, Na, P, S and Zn concentrations by introducing the sample into the plasma as an aerosol spray. The aerosol spray passes through the plasma torch which causes the atoms to enter an excited state. When this happens, the atoms emit light at their own characteristic wavelengths. The intensity of the light transmitted at these wavelengths is recorded to identify the specific elemental concentrations. We can detect P and K concentrations from 0.5% to 7.5% without a serial dilution (serial dilution increase concentration magnitude by six fold). We retest a sample with greater than a 3.25% K or P value before the data is approve

Skalar Wet Chemistry Analyzer

Skalar Wet Chemistry Instrument Principle of Operation
All media extracts, water, nutrient solutions, compost extracts and fertilizers are analyzed for NH4, NO3, Urea-N and Chloride on the SAN plus Segmented Flow Analyzer by Skalar Inc. It is an automated segmented flow instrument to measure nutrient concentrations by a continuous flow method of wet chemistry analysis. In this method a stream of reagents and sample are segmented with air bubbles and pumped through a manifold to undergo treatment such as mixing, heating or dialysis before entering a flow cell to be detected. After detection, the reaction mixture is transferred to a waste receptacle. The measure signals are collected on a computer, where the data and peaks are processed.

C/N/S Analyzer

Nitrogen Analysis--Understanding Carlo Erba Nitrogen and Sulfur Instruments
Instruments are gas chromatographs, separating elements for analysis through a gas form. Method is based on flash combustion: High temperature and injection of oxygen flow causes sample to become oxidized and combustion occurs, converting inorganic and organic samples into gas (and ash as a byproduct). Gases flow by carrier gas (helium), which is initially injected when auto sampler drops sample. Gases flow through copper (Cu) reduction tube, which reduces sulfur/nitrogen oxides to elemental nitrogen and sulfur. Gases flow into chromatographic column (GC column) inside furnace (metal-boxed area behind H2O trap) and are separated by thermal conductivity detector. This generates an electronic signal, reporting the concentration of nitrogen/sulfur found. A graph is displayed and samples are calculated for quantitative analysis. Excess gases are released through vents: M and R for carrier gas (helium) and O2 for oxygen.

Required Sample Amounts for Complete Analysis

Sample TypeAnalysisAmountDilution Factors
Tissue  10x100x
 % N4-7 mg  
 ICP Analysis0.5 g0.3 g0.2 g
 % S7-11 mg  
Media    
 ICP Analysis15 mL  
 Skalar Analysis20 mL  
 pH30 mL  
 EC5 mL  
Water    
 ICP Analysis15 mL   
 Skalar Analysis20 mL  
 pH30 mL  
 EC5 mL  
 Alkalinity60 mL  
Fertilizer Solution    
 ICP Analysis15 mL  
 Skalar Analysis20 mL  
 pH30 mL  
 EC5 mL  
 Alkalinity60 mL  
Dry Fertilizer    
 ICP Analysis15 mL  
 Skalar Analysis20 mL  
 % N15 mg  
What is...    
 ICP Analysis15 mL  
 Skalar Analysis20 mL  
 % N15 mg  

Copyright 2016 JR Peters Inc.